SPP382
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SQS1
Gene Ontology Biological Process
Gene Ontology Cellular Component
Dosage Lethality
A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.
Publication
Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function.
Prp43p catalyzes essential steps in pre-mRNA splicing and rRNA biogenesis. In splicing, Spp382p stimulates the Prp43p helicase to dissociate the postcatalytic spliceosome and, in some way, to maintain the integrity of the spliceosome assembly. Here we present a dosage interference assay to identify Spp382p-interacting factors by screening for genes that when overexpressed specifically inhibit the growth of a conditional lethal ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: viability (APO:0000111)
- phenotype: inviable (APO:0000112)
Additional Notes
- Overexpression of SQS1 is lethal in a PRP38/SPP382 double mutant
- genetic complex
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
SQS1 SPP382 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID