ATAD1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
USP14
Gene Ontology Biological Process
- negative regulation of ER-associated ubiquitin-dependent protein catabolic process [ISO]
- negative regulation of endopeptidase activity [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IBA, ISO]
- proteolysis [ISO]
- regulation of chemotaxis [ISO]
- regulation of proteasomal protein catabolic process [IBA, ISO]
- synaptic transmission [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The AAA-ATPase ATAD1 and its partners promote degradation of desmin intermediate filaments in muscle.
Maintenance of desmin intermediate filaments (IF) is vital for muscle plasticity and function, and their perturbed integrity due to accelerated loss or aggregation causes atrophy and myopathies. Calpain-1-mediated disassembly of ubiquitinated desmin IF is a prerequisite for desmin loss, myofibril breakdown, and atrophy. Because calpain-1 does not harbor a bona fide ubiquitin-binding domain, the precise mechanism for desmin IF disassembly ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID