Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

TIF1? inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex.

Su Z, Sun Z, Wang Z, Wang S, Wang Y, Jin E, Li C, Zhao J, Liu Z, Zhou Z, Wang Y, Chen X, Liu X, Lei Z, Zhang HT

The molecular underpinnings of lung adenocarcinoma (LUAD) metastasis remain poorly defined. Here, using human LUAD cell lines, we find that transcriptional intermediary factor 1 ? (TIF1?) binds to TATA box binding protein (TBP) in competition with TBP-associated factor 15 (TAF15) and impedes TAF15/TBP-mediated interleukin 6 (IL-6) transactivation. TIF1? modifies TAF15 through multi-mono-ubiquitylation and drives nuclear export of TAF15. Functionally, TAF15 ... [more]

Cell Rep Oct. 18, 2022; 41(3);111513 [Pubmed: 36261009]

Throughput

  • High Throughput

Curated By

  • BioGRID