Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Cysteine Modification by Ebselen Reduces the Stability and Cellular Levels of 14-3-3 Proteins.

Waloen K, Jung-Kc K, Vecchia ED, Pandey S, Gasparik N, Doskeland A, Patil S, Kleppe R, Hritz J, Norton WHJ, Martinez A, Haavik J

The 14-3-3 proteins constitute a family of adaptor proteins with many binding partners and biological functions, and they are considered promising drug targets in cancer and neuropsychiatry. By screening 1280 small-molecule drugs using differential scanning fluorimetry (DSF), we found 15 compounds that decreased the thermal stability of 14-3-3? Among these compounds, ebselen was identified as a covalent, destabilizing ligand of ... [more]

Mol Pharmacol Aug. 01, 2021; 100(2);155-169 [Pubmed: 34031189]

Throughput

  • Low Throughput

Curated By

  • BioGRID