BAIT

STIP1

HEL-S-94n, HOP, IEF-SSP-3521, P60, STI1, STI1L
stress-induced phosphoprotein 1
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

PSMC3

TBP1
proteasome (prosome, macropain) 26S subunit, ATPase, 3
GO Process (24)
GO Function (4)
GO Component (7)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The Hsp70-Hsp90 co-chaperone Hop/Stip1 shifts the proteostatic balance from folding towards degradation.

Bhattacharya K, Weidenauer L, Luengo TM, Pieters EC, Echeverria PC, Bernasconi L, Wider D, Sadian Y, Koopman MB, Villemin M, Bauer C, Ruediger SGD, Quadroni M, Picard D

Hop/Stip1/Sti1 is thought to be essential as a co-chaperone to facilitate substrate transfer between the Hsp70 and Hsp90 molecular chaperones. Despite this proposed key function for protein folding and maturation, it is not essential in a number of eukaryotes and bacteria lack an ortholog. We set out to identify and to characterize its eukaryote-specific function. Human cell lines and the ... [more]

Nat Commun Nov. 25, 2020; 11(1);5975 [Pubmed: 33239621]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PSMC3 STIP1
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.752BioGRID
754421

Curated By

  • BioGRID