An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A Degradation Motif in STAU1 Defines a Novel Family of Proteins Involved in Inflammation.

Gonzalez Quesada Y, DesGroseillers L

Cancer development is regulated by inflammation. Staufen1 (STAU1) is an RNA-binding protein whose expression level is critical in cancer cells as it is related to cell proliferation or cell death. STAU1 protein levels are downregulated during mitosis due to its degradation by the E3 ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). In this paper, we map the molecular determinant involved in STAU1 ... [more]

Int J Mol Sci Sep. 30, 2022; 23(19); [Pubmed: 36232890]

Throughput

High Throughput

Additional Notes

Proximity label-MS was carried out to identify high confidence protein interactors with a total spectrum count of greater than or equal to four times over control

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PPP1CC STAU1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Esteves SL (2012)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

STAU1

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

PPP1CC

Gene Ontology Biological Process

Gene Ontology Molecular Function

phosphatase activity [ISS]phosphoprotein phosphatase activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]protein serine/threonine phosphatase activity [IMP]