An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proximity-dependent biotin identification (BioID) reveals a dynamic LSD1-CoREST interactome during embryonic stem cell differentiation.

Barnes CE, English DM, Broderick M, Collins MO, Cowley SM

Lysine specific demethylase 1 (LSD1) regulates gene expression as part of the CoREST complex, along with co-repressor of REST (CoREST) and histone deacetylase 1 (HDAC1). CoREST is recruited to specific genomic loci by core components and numerous transient interactions with chromatin-associated factors and transcription factors. We hypothesise that many of these weaker and transient associations may be difficult to identify ... [more]

Mol Omics Jan. 17, 2022; 18(1);31-44 [Pubmed: 34709266]

Throughput

High Throughput

Additional Notes

BioID
HEK293T cells

Curated By

BioGRID

Interaction Summary

PHF21A

Gene Ontology Biological Process

Gene Ontology Cellular Component

UBR4

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin-protein transferase activity [IDA]