BAIT
GAP1
amino acid permease GAP1, L000000667, YKR039W
General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
GO Process (2)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
STP2
L000003370, YHR006W
Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication
GO Process (1)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Amino acid signaling in yeast: post-genome duplication divergence of the Stp1 and Stp2 transcription factors.
When yeast cells detect external amino acids via their permease-like Ssy1 sensor, the cytosolic precursor forms of Stp1 and Stp2 transcription factors are activated by endoproteolytic removal of their N-terminal domains, a reaction catalyzed by the Ssy5 endoprotease. The processed Stp factors then migrate into the nucleus, where they activate transcription of several amino acid permease genes including AGP1. We ... [more]
J. Biol. Chem. Jan. 08, 2010; 285(2);855-65 [Pubmed: 19906648]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
- phenotype: rna accumulation (APO:0000224)
Additional Notes
- GAP1/STP1/STP2 triple mutants are inviable
- genetic complex
- triple mutants show aberrant gene expression
Curated By
- BioGRID