POLR1A
Gene Ontology Biological Process
Gene Ontology Molecular Function
POLR2L
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [TAS]
- DNA repair [TAS]
- RNA splicing [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- mRNA splicing, via spliceosome [TAS]
- nucleotide-excision repair [TAS]
- positive regulation of type I interferon production [TAS]
- positive regulation of viral transcription [TAS]
- regulation of transcription from RNA polymerase I promoter [TAS]
- termination of RNA polymerase III transcription [TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription elongation from RNA polymerase III promoter [TAS]
- transcription from RNA polymerase I promoter [IBA]
- transcription from RNA polymerase II promoter [IDA, TAS]
- transcription from RNA polymerase III promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoans.
Transcription of the ribosomal RNA precursor by RNA polymerase (Pol) I is a major determinant of cellular growth, and dysregulation is observed in many cancer types. Here, we present the purification of human Pol I from cells carrying a genomic GFP fusion on the largest subunit allowing the structural and functional analysis of the enzyme across species. In contrast to ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| POLR1A POLR2L | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| POLR2L POLR1A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9996 | BioGRID | 3038354 | |
| POLR1A POLR2L | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 40.1004 | BioGRID | 2943272 | |
| POLR1A POLR2L | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| POLR2L POLR1A | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.2383 | BioGRID | 1266874 |
Curated By
- BioGRID