BAIT

CTF4

CHL15, POB1, chromatin-binding protein CTF4, L000000326, YPR135W
Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion
Saccharomyces cerevisiae (S288c)
PREY

DPB3

L000000520, YBR278W
Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Parental histone deposition on the replicated strands promotes error-free DNA damage tolerance and regulates drug resistance.

Dolce V, Dusi S, Giannattasio M, Joseph CR, Fumasoni M, Branzei D

Ctf4 is a conserved replisome component with multiple roles in DNA metabolism. To investigate connections between Ctf4-mediated processes involved in drug resistance, we conducted a suppressor screen of ctf4? sensitivity to the methylating agent MMS. We uncovered that mutations in Dpb3 and Dpb4 components of polymerase ? result in the development of drug resistance in ctf4? via their histone-binding function. ... [more]

Genes Dev Dec. 01, 2021; 36(3-4);167-179 [Pubmed: 35115379]

Throughput

  • Low Throughput

Ontology Terms

  • mutation frequency (APO:0000198)

Additional Notes

  • Figure 4
  • reversion rate

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DPB3 CTF4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1783BioGRID
360334
CTF4 DPB3
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
3531620

Curated By

  • BioGRID