diacylglycerol O-acyltransferase, YOR245C
Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor; Lro1p and Dga1p can O-acylate ceramides; localized to lipid particles
GO Process (3)
GO Function (1)
GO Component (2)
Saccharomyces cerevisiae (S288c)


PEM1, phosphatidylethanolamine N-methyltransferase, L000000328, YGR157W
Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.


Sterol and diacylglycerol acyltransferase deficiency triggers fatty acid-mediated cell death.

Garbarino J, Padamsee M, Wilcox L, Oelkers PM, D'Ambrosio D, Ruggles KV, Ramsey N, Jabado O, Turkish A, Sturley SL

Deletion of the acyltransferases responsible for triglyceride and steryl ester synthesis in Saccharomyces cerevisiae serves as a genetic model of diseases where lipid overload is a component. The yeast mutants lack detectable neutral lipids and cytoplasmic lipid droplets and are strikingly sensitive to unsaturated fatty acids. Expression of human diacylglycerol acyltransferase 2 in the yeast mutants was sufficient to reverse ... [more]

J. Biol. Chem. Nov. 06, 2009; 284(45);30994-1005 [Pubmed: 19690167]


  • Low Throughput

Ontology Terms

  • phenotype: rna accumulation (APO:0000224)
  • phenotype: protein transport (APO:0000129)
  • phenotype: inviable (APO:0000112)
  • phenotype: mating efficiency (APO:0000034)

Additional Notes

  • DGA1/LRO1/CHO2 triple mutants are synthetic lethal
  • genetic complex

Curated By

  • BioGRID