YWHAQ
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
WWTR1
Gene Ontology Biological Process
- gene expression [TAS]
- hippo signaling [IDA, TAS]
- negative regulation of canonical Wnt signaling pathway [IMP]
- negative regulation of catenin import into nucleus [IMP]
- negative regulation of protein kinase activity [IMP]
- negative regulation of protein phosphorylation [IMP]
- positive regulation of cell proliferation [IDA]
- positive regulation of epithelial to mesenchymal transition [IDA]
- positive regulation of transcription from RNA polymerase II promoter [TAS]
- regulation of SMAD protein import into nucleus [IDA]
- regulation of transcription, DNA-templated [NAS]
- stem cell division [IDA]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A central chaperone-like role for 14-3-3 proteins in human cells.
14-3-3 proteins are highly conserved regulatory proteins that interact with hundreds of structurally diverse clients and act as central hubs of signaling networks. However, how 14-3-3 paralogs differ in specificity and how they regulate client protein function are not known for most clients. Here, we map the interactomes of all human 14-3-3 paralogs and systematically characterize the effect of disrupting ... [more]
Throughput
- High Throughput
Additional Notes
- Interaction confidence score is 1 minus the Bayesian False Discovery Rate (BFDR) calculated from SAINT analysis. Interaction cutoff is BFDR <= 0.01 (confidence score >= 0.99)
- Vehicle control condition
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| WWTR1 YWHAQ | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3225441 | |
| YWHAQ WWTR1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9873 | BioGRID | 3137943 | |
| WWTR1 YWHAQ | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 727621 |
Curated By
- BioGRID