Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Myosin-X recruits lamellipodin to filopodia tips.

Popovic A, Miihkinen M, Ghimire S, Saup R, Groenloh MLB, Ball NJ, Goult BT, Ivaska J, Jacquemet G

Myosin-X (MYO10), a molecular motor localizing to filopodia, is thought to transport various cargo to filopodia tips, modulating filopodia function. However, only a few MYO10 cargoes have been described. Here, using GFP-Trap and BioID approaches combined with mass spectrometry, we identified lamellipodin (RAPH1) as a novel MYO10 cargo. We report that the FERM domain of MYO10 is required for RAPH1 ... [more]

J Cell Sci Mar. 01, 2023; 136(5); [Pubmed: 36861887]

Throughput

  • High Throughput

Curated By

  • BioGRID