Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Cell cycle-dependent palmitoylation of protocadherin 7 by ZDHHC5 promotes successful cytokinesis.

Ozkan Kucuk NE, Yigit BN, Degirmenci BS, Qureshi MH, Yapici GN, Kamacioglu A, Bavili N, Kiraz A, Ozlu N

Cell division requires dramatic reorganization of the cell cortex, which is primarily driven by the actomyosin network. We previously reported that protocadherin 7 (PCDH7) gets enriched at the cell surface during mitosis, which is required to build up the full mitotic rounding pressure. Here, we report that PCDH7 interacts with and is palmitoylated by the palmitoyltransferase, ZDHHC5. PCDH7 and ZDHHC5 ... [more]

J Cell Sci Mar. 15, 2023; 136(6); [Pubmed: 36762613]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • false discovery rate (FDR) of 0.05

Curated By

  • BioGRID