Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A central chaperone-like role for 14-3-3 proteins in human cells.

Segal D, Maier S, Mastromarco GJ, Qian WW, Nabeel-Shah S, Lee H, Moore G, Lacoste J, Larsen B, Lin ZY, Selvabaskaran A, Liu K, Smibert C, Zhang Z, Greenblatt J, Peng J, Lee HO, Gingras AC, Taipale M

14-3-3 proteins are highly conserved regulatory proteins that interact with hundreds of structurally diverse clients and act as central hubs of signaling networks. However, how 14-3-3 paralogs differ in specificity and how they regulate client protein function are not known for most clients. Here, we map the interactomes of all human 14-3-3 paralogs and systematically characterize the effect of disrupting ... [more]

Mol Cell Mar. 16, 2023; 83(6);974-993.e15 [Pubmed: 36931259]

Throughput

  • High Throughput

Additional Notes

  • Interaction confidence score is 1 minus the Bayesian False Discovery Rate (BFDR) calculated from SAINT analysis. Interaction cutoff is BFDR <= 0.01 (confidence score >= 0.99)
  • okadaic acid (serine/threonine phosphatases inhibition) condition

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IRS4 YWHAQ
Affinity Capture-Luminescence
Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Low-BioGRID
-
IRS4 YWHAQ
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
YWHAQ IRS4
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3535669

Curated By

  • BioGRID