Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A central chaperone-like role for 14-3-3 proteins in human cells.

Segal D, Maier S, Mastromarco GJ, Qian WW, Nabeel-Shah S, Lee H, Moore G, Lacoste J, Larsen B, Lin ZY, Selvabaskaran A, Liu K, Smibert C, Zhang Z, Greenblatt J, Peng J, Lee HO, Gingras AC, Taipale M

14-3-3 proteins are highly conserved regulatory proteins that interact with hundreds of structurally diverse clients and act as central hubs of signaling networks. However, how 14-3-3 paralogs differ in specificity and how they regulate client protein function are not known for most clients. Here, we map the interactomes of all human 14-3-3 paralogs and systematically characterize the effect of disrupting ... [more]

Mol Cell Mar. 16, 2023; 83(6);974-993.e15 [Pubmed: 36931259]

Throughput

  • High Throughput

Additional Notes

  • Interaction confidence score is 1 minus the Bayesian False Discovery Rate (BFDR) calculated from SAINT analysis. Interaction cutoff is BFDR <= 0.01 (confidence score >= 0.99)
  • okadaic acid (serine/threonine phosphatases inhibition) condition

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YWHAQ SORBS2
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3535771

Curated By

  • BioGRID