An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Evidence for hSNM1B/Apollo functioning in the HSP70 mediated DNA damage response.

Anders M, Mattow J, Digweed M, Demuth I

The hSNM1B/Apollo protein is involved in the cellular response to DNA-damage as well as in the maintenance of telomeres during S-phase. TRF2 has been shown to interact physically with hSNM1B. As a core component of shelterin, TRF2 functions in organization and protection of telomeres. However, TRF2 was also shown to have a role in the early DNA-damage response, suggesting that ... [more]

Cell Cycle Jun. 01, 2009; 8(11);1725-32 [Pubmed: 19411856]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

DCLRE1B

Gene Ontology Biological Process

Gene Ontology Molecular Function

5'-3' exonuclease activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

HSPA4