BAIT

GSK3B

glycogen synthase kinase 3 beta
GO Process (35)
GO Function (11)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Interactomes of Glycogen Synthase Kinase-3 Isoforms.

Cormier KW, Larsen B, Gingras AC, Woodgett JR

Functional differentiation of the two isoforms of the protein-serine/threonine kinase, glycogen synthase kinase-3 (GSK-3), is an unsettled area of research. The isoforms are highly similar in structure and are largely redundant, though there is also evidence for specific roles. Identification of isoform-specific protein interactors may elucidate the differences in function and provide insight into isoform-selective regulation. We therefore sought to ... [more]

J Proteome Res Feb. 13, 2023; (); [Pubmed: 36779422]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • HeLa cells
  • SAINT cutoff corresponding to a protein-level Bayesian false discovery rate (BFDR) <= 1%

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GSK3B VCL
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID