BAIT

SOH1

MED31, L000001982, YGL127C
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
GO Process (5)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SUB1

TSP1, L000003936, YMR039C
Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in nonhomologous end-joining (NHEJ) of ds breaks in plasmid DNA, but not chromosomal DNA; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Mediator subunits and histone methyltransferase Set2 contribute to Ino2-dependent transcriptional activation of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae.

Dettmann A, Jaeschke Y, Triebel I, Bogs J, Schroeder I, Schueller HJ

To activate eukaryotic genes, several pathways which modify chromatin and recruit general factors of the transcriptional machinery are utilized. We investigated the factors required for activation of yeast phospholipid biosynthetic genes, depending on activator protein Ino2 which binds to the inositol/choline-responsive element (ICRE) upstream promoter motif together with its partner protein Ino4. We used a set of 15 strains each ... [more]

Mol. Genet. Genomics Mar. 01, 2010; 283(3);211-21 [Pubmed: 20054697]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: stress resistance (APO:0000080)
  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • The double mutant showed a synthetic growth defect in medium lacking inositol + choline (IC).

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SOH1 SUB1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-4.8856BioGRID
509174

Curated By

  • BioGRID