BAIT

FOXO1

FKH1, FKHR, FOXO1A
forkhead box O1
GO Process (30)
GO Function (6)
GO Component (5)

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Study of FOXO1-interacting proteins using TurboID-based proximity labeling technology.

Su Y, Guo Y, Guo J, Zeng T, Wang T, Liu W

Protein?protein interactions (PPIs) are the foundation of the life activities of cells. TurboID is a biotin ligase with higher catalytic efficiency than BioID or APEX that reduces the required labeling time from 18 h to 10 min. Since many proteins participate in binding and catalytic events that are very short-lived, it is theoretically possible to find relatively novel binding proteins using the ... [more]

BMC Genomics Mar. 24, 2023; 24(1);146 [Pubmed: 36964488]

Throughput

  • High Throughput

Additional Notes

  • FOXO1 overexpression group
  • LPS treatment group
  • TurboID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FOXO1 HNRNPK
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
3539037

Curated By

  • BioGRID