PREY

RGL2

KE1.5, Rab2l, Rgt2, Rlf
ral guanine nucleotide dissociation stimulator-like 2
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Structure determination of the Ras-binding domain of the Ral-specific guanine nucleotide exchange factor Rlf.

Esser D, Bauer B, Wolthuis RM, Wittinghofer A, Cool RH, Bayer P

Ral-specific guanine nucleotide exchange factors RalGDS, Rgl, and Rlf have been suggested to function as intermediates between Ras and Ral pathways by being able to bind Ras proteins through their C-terminal Ras-binding domains (RBD). The RBDs of RalGDS and of the Ser/Thr kinase c-Raf-1 have been shown to have the same tertiary structure. In contrast to the RBDs of Raf ... [more]

Biochemistry Sep. 29, 1998; 37(39);13453-62 [Pubmed: 9753431]

Throughput

  • Low Throughput

Curated By

  • BioGRID