BAIT

ARNT

HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2
aryl hydrocarbon receptor nuclear translocator
Homo sapiens
PREY

CLOCK

KAT13D, bHLHe8
clock circadian regulator
GO Process (20)
GO Function (11)
GO Component (7)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The basic-helix-loop-helix-PAS orphan MOP3 forms transcriptionally active complexes with circadian and hypoxia factors.

Hogenesch JB, Gu YZ, Jain S, Bradfield CA

We report that MOP3 is a general dimerization partner for a subset of the basic-helix-loop-helix (bHLH)-PER-ARNT-SIM (PAS) superfamily of transcriptional regulators. We demonstrated that MOP3 interacts with MOP4, CLOCK, hypoxia-inducible factor 1alpha (HIF1alpha), and HIF2alpha. A DNA selection protocol revealed that the MOP3-MOP4 heterodimer bound a CACGTGA-containing DNA element. Transient transfection experiments demonstrated that the MOP3-MOP4 and MOP3-CLOCK complexes bound ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 12, 1998; 95(10);5474-9 [Pubmed: 9576906]

Throughput

  • Low Throughput

Curated By

  • BioGRID