Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Regulation of RNA editing by RNA-binding proteins in human cells.

Quinones-Valdez G, Tran SS, Jun HI, Bahn JH, Yang EW, Zhan L, Bruemmer A, Wei X, Van Nostrand EL, Pratt GA, Yeo GW, Graveley BR, Xiao X

Adenosine-to-inosine (A-to-I) editing, mediated by the ADAR enzymes, diversifies the transcriptome by altering RNA sequences. Recent studies reported global changes in RNA editing in disease and development. Such widespread editing variations necessitate an improved understanding of the regulatory mechanisms of RNA editing. Here, we study the roles of?>200 RNA-binding proteins (RBPs) in mediating RNA editing in two human cell lines. ... [more]

Commun Biol Jan. 18, 2019; 2();19 [Pubmed: 30652130]

Throughput

  • Low Throughput

Curated By

  • BioGRID