CHTOP
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PRMT1
Gene Ontology Biological Process
- histone H4-R3 methylation [IBA, ISO]
- histone methylation [IDA, ISO]
- in utero embryonic development [IMP]
- negative regulation of megakaryocyte differentiation [ISO]
- neuron projection development [ISO]
- peptidyl-arginine N-methylation [IDA]
- peptidyl-arginine methylation [ISO]
- peptidyl-arginine methylation, to asymmetrical-dimethyl arginine [IBA, ISO]
- peptidyl-arginine omega-N-methylation [ISO]
- protein methylation [IMP, ISO]
- regulation of transcription, DNA-templated [IBA]
Gene Ontology Molecular Function- N-methyltransferase activity [ISO]
- [cytochrome c]-arginine N-methyltransferase activity [ISO]
- histone methyltransferase activity [IDA, ISO]
- histone methyltransferase activity (H4-R3 specific) [IBA, ISO]
- histone-arginine N-methyltransferase activity [ISO]
- identical protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein methyltransferase activity [IMP, ISO]
- protein-arginine N-methyltransferase activity [IDA, ISO]
- protein-arginine omega-N asymmetric methyltransferase activity [IBA, ISO]
- protein-arginine omega-N monomethyltransferase activity [ISO]
- snoRNP binding [ISO]
- N-methyltransferase activity [ISO]
- [cytochrome c]-arginine N-methyltransferase activity [ISO]
- histone methyltransferase activity [IDA, ISO]
- histone methyltransferase activity (H4-R3 specific) [IBA, ISO]
- histone-arginine N-methyltransferase activity [ISO]
- identical protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein methyltransferase activity [IMP, ISO]
- protein-arginine N-methyltransferase activity [IDA, ISO]
- protein-arginine omega-N asymmetric methyltransferase activity [IBA, ISO]
- protein-arginine omega-N monomethyltransferase activity [ISO]
- snoRNP binding [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.
Chromatin target of Prmt1 (Chtop) is a vertebrate-specific chromatin-bound protein that plays an important role in transcriptional regulation. As its mechanism of action remains unclear, we identified Chtop-interacting proteins using a biotinylation-proteomics approach. Here we describe the identification and initial characterization of Five Friends of Methylated Chtop (5FMC). 5FMC is a nuclear complex that can only be recruited by Chtop ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CHTOP PRMT1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID