ZFP148
Gene Ontology Biological Process
- gamete generation [IMP]
- negative regulation of gene expression [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein complex assembly [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- double-stranded DNA binding [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region sequence-specific DNA binding [ISO]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- double-stranded DNA binding [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region sequence-specific DNA binding [ISO]
Gene Ontology Cellular Component
WDR18
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.
Chromatin target of Prmt1 (Chtop) is a vertebrate-specific chromatin-bound protein that plays an important role in transcriptional regulation. As its mechanism of action remains unclear, we identified Chtop-interacting proteins using a biotinylation-proteomics approach. Here we describe the identification and initial characterization of Five Friends of Methylated Chtop (5FMC). 5FMC is a nuclear complex that can only be recruited by Chtop ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID