BAIT

ENP1

MEG1, L000003200, L000001059, YBR247C
Protein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
Saccharomyces cerevisiae (S288c)
PREY

RPL20B

RPL18A1, ribosomal 60S subunit protein L20B, L20e, L20B, L18B, L000003148, YOR312C
Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Bud23 promotes the final disassembly of the small subunit Processome in Saccharomyces cerevisiae.

Black JJ, Sardana R, Elmir EW, Johnson AW

The first metastable assembly intermediate of the eukaryotic ribosomal small subunit (SSU) is the SSU Processome, a large complex of RNA and protein factors that is thought to represent an early checkpoint in the assembly pathway. Transition of the SSU Processome towards continued maturation requires the removal of the U3 snoRNA and biogenesis factors as well as ribosomal RNA processing. ... [more]

PLoS Genet Dec. 01, 2020; 16(12);e1009215 [Pubmed: 33306676]

Throughput

  • High Throughput

Curated By

  • BioGRID