Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.

Navare AT, Mast FD, Olivier JP, Bertomeu T, Neal ML, Carpp LN, Kaushansky A, Coulombe-Huntington J, Tyers M, Aitchison JD

Viruses co-opt host proteins to carry out their lifecycle. Repurposed host proteins may thus become functionally compromised; a situation analogous to a loss-of-function mutation. We term such host proteins as viral-induced hypomorphs. Cells bearing cancer driver loss-of-function mutations have successfully been targeted with drugs perturbing proteins encoded by the synthetic lethal (SL) partners of cancer-specific mutations. Similarly, SL interactions of ... [more]

J Cell Biol Nov. 07, 2022; 221(11); [Pubmed: 36305789]

Throughput

Low Throughput

Ontology Terms

growth abnormality (HP:0001507) [viability (PATO:0000169)]

Additional Notes

CRISPR GI screen
Cell Line: NALM-6
Experimental Setup: Negative Genetic (Synthetic Lethal)
GIST: A-phenotypic negative genetic interaction
Hit genes are synthetic lethal with chemical inhibition of GBF1 using golgicide A (GCA)
Library: EKO (Extended Knockout Library)
Significance Threshold: FDR<0.05

Curated By

BioGRID

Interaction Summary

GBF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ARF guanyl-nucleotide exchange factor activity [IBA]protein binding [IPI]

Gene Ontology Cellular Component

RAB3GAP2

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [IPI]Rab guanyl-nucleotide exchange factor activity [IMP]enzyme activator activity [TAS]enzyme regulator activity [ISS]protein heterodimerization activity [ISS]