BAIT
GBF1
ARF1GEF
golgi brefeldin A resistant guanine nucleotide exchange factor 1
GO Process (6)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PDIA6
ERP5, P5, TXNDC7
protein disulfide isomerase family A, member 6
GO Process (6)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.
Viruses co-opt host proteins to carry out their lifecycle. Repurposed host proteins may thus become functionally compromised; a situation analogous to a loss-of-function mutation. We term such host proteins as viral-induced hypomorphs. Cells bearing cancer driver loss-of-function mutations have successfully been targeted with drugs perturbing proteins encoded by the synthetic lethal (SL) partners of cancer-specific mutations. Similarly, SL interactions of ... [more]
J Cell Biol Nov. 07, 2022; 221(11); [Pubmed: 36305789]
Throughput
- Low Throughput
Ontology Terms
- growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line: NALM-6
- Experimental Setup: Negative Genetic (Synthetic Lethal)
- GIST: A-phenotypic negative genetic interaction
- Hit genes are synthetic lethal with chemical inhibition of GBF1 using golgicide A (GCA)
- Library: EKO (Extended Knockout Library)
- Significance Threshold: FDR<0.05
Curated By
- BioGRID