GBF1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ARFGAP1
Gene Ontology Biological Process
- COPI coating of Golgi vesicle [TAS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- cellular protein metabolic process [TAS]
- endoplasmic reticulum unfolded protein response [TAS]
- membrane organization [TAS]
- positive regulation of GTPase activity [IDA]
- retrograde vesicle-mediated transport, Golgi to ER [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.
Viruses co-opt host proteins to carry out their lifecycle. Repurposed host proteins may thus become functionally compromised; a situation analogous to a loss-of-function mutation. We term such host proteins as viral-induced hypomorphs. Cells bearing cancer driver loss-of-function mutations have successfully been targeted with drugs perturbing proteins encoded by the synthetic lethal (SL) partners of cancer-specific mutations. Similarly, SL interactions of ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line: NALM-6
- Experimental Setup: Positive Genetic (Suppressor)
- GIST: A-phenotypic positive genetic interaction
- Hit genes are suppress growth inhibition due to chemical inhibition of GBF1 using golgicide A (GCA)
- Library: EKO (Extended Knockout Library)
- Significance Threshold: FDR<0.05
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GBF1 ARFGAP1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 2616233 |
Curated By
- BioGRID