BAIT
ADAM10
1700031C13Rik, MADM, kuz, kuzbanian, RP24-68C7.1
a disintegrin and metallopeptidase domain 10
GO Process (16)
GO Function (7)
GO Component (15)
Gene Ontology Biological Process
- Notch receptor processing [NAS]
- Notch signaling pathway [IMP]
- PMA-inducible membrane protein ectodomain proteolysis [ISO]
- constitutive protein ectodomain proteolysis [ISO]
- in utero embryonic development [IMP]
- membrane protein ectodomain proteolysis [ISO]
- monocyte activation [ISO]
- negative regulation of cell adhesion [ISO]
- nucleocytoplasmic transport [NAS]
- positive regulation of T cell chemotaxis [ISO]
- positive regulation of cell growth [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of cell proliferation [ISO]
- protein phosphorylation [IDA]
- proteolysis [IMP]
- response to tumor necrosis factor [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- Golgi-associated vesicle [ISO]
- cell [NAS]
- cell surface [ISO]
- cytoplasm [IDA]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- intracellular [NAS]
- intracellular membrane-bounded organelle [ISO]
- membrane [ISO]
- nucleus [IDA]
- perinuclear endoplasmic reticulum [ISO]
- postsynaptic density [IDA]
- tetraspanin-enriched microdomain [ISO]
- trans-Golgi network [ISO]
Mus musculus
PREY
NCKAP1
C79304, H19, Hem-2, Hem2, Nap1, mKIAA0587, mh19, p125Nap1, RP23-33L19.3
NCK-associated protein 1
GO Process (23)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- Rac protein signal transduction [ISO]
- apical protein localization [IDA]
- basal protein localization [IDA]
- cell migration involved in gastrulation [IMP]
- embryonic body morphogenesis [IMP]
- embryonic foregut morphogenesis [IMP]
- embryonic heart tube development [IMP]
- endoderm development [IMP]
- establishment or maintenance of actin cytoskeleton polarity [IMP]
- in utero embryonic development [IMP]
- lamellipodium assembly [IDA, IMP]
- mesodermal cell migration [IMP]
- neural tube closure [IMP]
- notochord development [IMP]
- notochord morphogenesis [IMP]
- paraxial mesoderm development [IMP]
- paraxial mesoderm morphogenesis [IMP]
- positive regulation of Arp2/3 complex-mediated actin nucleation [ISO]
- positive regulation of lamellipodium assembly [ISO]
- protein stabilization [IMP]
- regulation of protein localization [IMP]
- somitogenesis [IMP]
- zygotic determination of anterior/posterior axis, embryo [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
ADAM10 hyperactivation acts on piccolo to deplete synaptic vesicle stores in Huntington's disease.
Synaptic dysfunction and cognitive decline in Huntington's disease (HD) involve hyperactive A disintegrin and metalloproteinase domain-containing protein 10 (ADAM10). To identify the molecular mechanisms through which ADAM10 is associated with synaptic dysfunction in HD, we performed an immunoaffinity purification-mass spectrometry (IP-MS) study of endogenous ADAM10 in the brains of wild-type and HD mice. We found that proteins implicated in synapse ... [more]
Hum Mol Genet Jun. 17, 2021; 30(13);1175-1187 [Pubmed: 33601422]
Throughput
- Low Throughput
Curated By
- BioGRID