BAIT

ADAM10

1700031C13Rik, MADM, kuz, kuzbanian, RP24-68C7.1

a disintegrin and metallopeptidase domain 10

GO Process (16)

GO Function (7)

GO Component (15)

Gene Ontology Molecular Function

SH2 domain binding [NAS]
endopeptidase activity [IDA]
metalloendopeptidase activity [ISO]
metallopeptidase activity [IDA, IMP, ISO]
protein binding [IPI]
protein homodimerization activity [IPI]
protein kinase binding [IDA]

Gene Ontology Cellular Component

Golgi apparatus [ISO]

Golgi-associated vesicle [ISO]

cell surface [ISO]

cytoplasm [IDA]

extracellular vesicular exosome [ISO]

focal adhesion [ISO]

intracellular [NAS]

intracellular membrane-bounded organelle [ISO]

perinuclear endoplasmic reticulum [ISO]

postsynaptic density [IDA]

tetraspanin-enriched microdomain [ISO]

trans-Golgi network [ISO]

CRISPR Database MGI Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

PREY

TPR

2610029M07Rik, C77892

translocated promoter region, nuclear basket protein

GO Process (18)

GO Function (10)

GO Component (11)

Gene Ontology Biological Process

MAPK import into nucleus [ISO]

RNA export from nucleus [ISO]

RNA import into nucleus [ISO]

cellular response to heat [ISO]

cellular response to interferon-alpha [IDA]

mRNA export from nucleus in response to heat stress [ISO]

mitotic spindle assembly checkpoint [ISO]

negative regulation of RNA export from nucleus [ISO]

negative regulation of transcription from RNA polymerase II promoter [ISO]

negative regulation of translational initiation [ISO]

positive regulation of heterochromatin assembly [ISO]

positive regulation of intracellular protein transport [ISO]

positive regulation of mitotic cell cycle spindle assembly checkpoint [ISO]

positive regulation of protein export from nucleus [ISO]

positive regulation of protein import into nucleus [ISO]

regulation of mitotic sister chromatid separation [ISO]

regulation of spindle assembly involved in mitosis [ISO]

response to epidermal growth factor [ISO]

Gene Ontology Molecular Function

chromatin binding [ISO]
dynein complex binding [ISO]
heat shock protein binding [ISO]
mRNA binding [ISO]
mitogen-activated protein kinase binding [ISO]
nucleocytoplasmic transporter activity [ISO]
poly(A) RNA binding [ISO]
protein binding [IPI]
protein homodimerization activity [ISO]
tubulin binding [ISO]

Gene Ontology Cellular Component

cytoplasm [ISO]

cytoplasmic dynein complex [ISO]

extrinsic component of membrane [ISO]

mitotic spindle [ISO]

nuclear envelope [IDA, ISO]

nuclear inclusion body [IDA, ISO]

nuclear membrane [ISO]

nuclear periphery [IDA, ISO]

nuclear pore [IDA, ISO]

nuclear pore nuclear basket [ISO]

CRISPR Database MGI Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

ADAM10 hyperactivation acts on piccolo to deplete synaptic vesicle stores in Huntington's disease.

Cozzolino F, Vezzoli E, Cheroni C, Besusso D, Conforti P, Valenza M, Iacobucci I, Monaco V, Birolini G, Bombaci M, Falqui A, Saftig P, Rossi RL, Monti M, Cattaneo E, Zuccato C

Synaptic dysfunction and cognitive decline in Huntington's disease (HD) involve hyperactive A disintegrin and metalloproteinase domain-containing protein 10 (ADAM10). To identify the molecular mechanisms through which ADAM10 is associated with synaptic dysfunction in HD, we performed an immunoaffinity purification-mass spectrometry (IP-MS) study of endogenous ADAM10 in the brains of wild-type and HD mice. We found that proteins implicated in synapse ... [more]

Hum Mol Genet Jun. 17, 2021; 30(13);1175-1187 [Pubmed: 33601422]

Throughput

Low Throughput

Curated By

BioGRID