Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A novel p53 regulator, C16ORF72/TAPR1, buffers against telomerase inhibition.

Benslimane Y, Sanchez-Osuna M, Coulombe-Huntington J, Bertomeu T, Henry D, Huard C, Bonneil E, Thibault P, Tyers M, Harrington L

Telomere erosion in cells with insufficient levels of the telomerase reverse transcriptase (TERT), contributes to age-associated tissue dysfunction and senescence, and p53 plays a crucial role in this response. We undertook a genome-wide CRISPR screen to identify gene deletions that sensitized p53-positive human cells to telomerase inhibition. We uncovered a previously unannotated gene, C16ORF72, which we term Telomere Attrition and ... [more]

Aging Cell Apr. 01, 2021; 20(4);e13331 [Pubmed: 33660365]

Throughput

High Throughput

Ontology Terms

growth abnormality (HP:0001507) [viability (PATO:0000169)]

Additional Notes

CRISPR GI screen
Cell Line: NALM-6
Experimental Setup: Timecourse
GIST: A-phenotypic negative genetic interaction
Library: TKOv3
Significance Threshold: <-4.1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
C16ORF72 CSNK2A2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Monda JK (2023)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

C16ORF72

CSNK2A2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein N-terminus binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

Negative Genetic