Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins.

Yang J, Guo F, Chin HS, Chen GB, Ang CH, Lin Q, Hong W, Fu NY

Tetraspanins, a superfamily of membrane proteins, mediate diverse biological processes through tetraspanin-enriched microdomains in the plasma membrane. However, how their cell-surface presentation is controlled remains unclear. To identify the regulators of tetraspanin trafficking, we conduct sequential genome-wide loss-of-function CRISPR-Cas9 screens based on cell-surface expression of a tetraspanin member, TSPAN8. Several genes potentially involved in endoplasmic reticulum (ER) targeting, different biological ... [more]

Cell Rep Jan. 31, 2023; 42(2);112065 [Pubmed: 36724073]

Throughput

High Throughput

Ontology Terms

protein/peptide accumulation (APO:0000149)

Additional Notes

CRISPR GI screen
Cell Line: MEC
Experimental Setup: Timecourse
GIST: A-phenotypic positive genetic interaction
Library: Human Two Plasmid Activity-Optimized CRISPR Knockout Library (ADDGENE:1000000095)
Significance Threshold: Candidate target genes with p < 0.01 from the rescue CRISPR-Cas9 screen on MEC SPPL3 KO cells

Curated By

BioGRID

Interaction Summary

SPPL3

Gene Ontology Biological Process

Gene Ontology Molecular Function

aspartic endopeptidase activity, intramembrane cleaving [IMP]protein homodimerization activity [IDA]

Gene Ontology Cellular Component

PARP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NAD+ ADP-ribosyltransferase activity [IDA, TAS]enzyme binding [IPI]identical protein binding [IPI]poly(A) RNA binding [IDA]protein N-terminus binding [IPI]protein binding [IPI]transcription factor binding [IPI]