SPPL3
Gene Ontology Biological Process
Gene Ontology Molecular Function
RPL11
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- protein localization to nucleus [ISS]
- protein targeting [IMP]
- rRNA processing [IMP]
- ribosomal large subunit biogenesis [IMP]
- translation [NAS, TAS]
- translational elongation [TAS]
- translational initiation [TAS]
- translational termination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral transcription [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins.
Tetraspanins, a superfamily of membrane proteins, mediate diverse biological processes through tetraspanin-enriched microdomains in the plasma membrane. However, how their cell-surface presentation is controlled remains unclear. To identify the regulators of tetraspanin trafficking, we conduct sequential genome-wide loss-of-function CRISPR-Cas9 screens based on cell-surface expression of a tetraspanin member, TSPAN8. Several genes potentially involved in endoplasmic reticulum (ER) targeting, different biological ... [more]
Throughput
- High Throughput
Ontology Terms
- protein/peptide accumulation (APO:0000149)
Additional Notes
- CRISPR GI screen
- Cell Line: MEC
- Experimental Setup: Timecourse
- GIST: A-phenotypic positive genetic interaction
- Library: Human Two Plasmid Activity-Optimized CRISPR Knockout Library (ADDGENE:1000000095)
- Significance Threshold: Candidate target genes with p < 0.01 from the rescue CRISPR-Cas9 screen on MEC SPPL3 KO cells
Curated By
- BioGRID