Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins.

Yang J, Guo F, Chin HS, Chen GB, Ang CH, Lin Q, Hong W, Fu NY

Tetraspanins, a superfamily of membrane proteins, mediate diverse biological processes through tetraspanin-enriched microdomains in the plasma membrane. However, how their cell-surface presentation is controlled remains unclear. To identify the regulators of tetraspanin trafficking, we conduct sequential genome-wide loss-of-function CRISPR-Cas9 screens based on cell-surface expression of a tetraspanin member, TSPAN8. Several genes potentially involved in endoplasmic reticulum (ER) targeting, different biological ... [more]

Cell Rep Jan. 31, 2023; 42(2);112065 [Pubmed: 36724073]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: protein/peptide accumulation (APO:0000149)

Additional Notes

  • CRISPR GI screen
  • Cell Line: MEC
  • Experimental Setup: Timecourse
  • GIST: A-phenotypic positive genetic interaction
  • Library: Human Two Plasmid Activity-Optimized CRISPR Knockout Library (ADDGENE:1000000095)
  • Significance Threshold: Candidate target genes with p < 0.01 from the rescue CRISPR-Cas9 screen on MEC SPPL3 KO cells

Curated By

  • BioGRID