BAIT

SPPL3

IMP2, MDHV1887, PRO4332, PSH1, PSL4, UNQ1887
signal peptide peptidase like 3
Homo sapiens
PREY

GJB6

CX30, DFNA3, DFNA3B, DFNB1B, ECTD2, ED2, EDH, HED, HED2
gap junction protein, beta 6, 30kDa
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins.

Yang J, Guo F, Chin HS, Chen GB, Ang CH, Lin Q, Hong W, Fu NY

Tetraspanins, a superfamily of membrane proteins, mediate diverse biological processes through tetraspanin-enriched microdomains in the plasma membrane. However, how their cell-surface presentation is controlled remains unclear. To identify the regulators of tetraspanin trafficking, we conduct sequential genome-wide loss-of-function CRISPR-Cas9 screens based on cell-surface expression of a tetraspanin member, TSPAN8. Several genes potentially involved in endoplasmic reticulum (ER) targeting, different biological ... [more]

Cell Rep Jan. 31, 2023; 42(2);112065 [Pubmed: 36724073]

Throughput

  • High Throughput

Ontology Terms

  • protein/peptide accumulation (APO:0000149)

Additional Notes

  • CRISPR GI screen
  • Cell Line: MEC
  • Experimental Setup: Timecourse
  • GIST: A-phenotypic positive genetic interaction
  • Library: Human Two Plasmid Activity-Optimized CRISPR Knockout Library (ADDGENE:1000000095)
  • Significance Threshold: Candidate target genes with p < 0.01 from the rescue CRISPR-Cas9 screen on MEC SPPL3 KO cells

Curated By

  • BioGRID