SPPL3
Gene Ontology Biological Process
Gene Ontology Molecular Function
FAP
Gene Ontology Biological Process
- endothelial cell migration [IDA]
- melanocyte apoptotic process [ISS]
- melanocyte proliferation [ISS]
- mitotic cell cycle arrest [ISS]
- negative regulation of cell proliferation involved in contact inhibition [ISS]
- negative regulation of extracellular matrix disassembly [IDA]
- negative regulation of extracellular matrix organization [IDA]
- positive regulation of cell cycle arrest [ISS]
- positive regulation of execution phase of apoptosis [ISS]
- proteolysis [IDA]
- proteolysis involved in cellular protein catabolic process [IDA]
- regulation of collagen catabolic process [IDA]
- regulation of fibrinolysis [IC]
Gene Ontology Molecular Function- dipeptidyl-peptidase activity [IDA, NAS]
- endopeptidase activity [IDA]
- integrin binding [IPI]
- metalloendopeptidase activity [TAS]
- peptidase activity [IDA]
- protease binding [IPI]
- protein binding [IPI]
- protein dimerization activity [NAS]
- protein homodimerization activity [IDA, NAS]
- serine-type endopeptidase activity [IDA]
- serine-type peptidase activity [IDA, IMP, NAS]
- dipeptidyl-peptidase activity [IDA, NAS]
- endopeptidase activity [IDA]
- integrin binding [IPI]
- metalloendopeptidase activity [TAS]
- peptidase activity [IDA]
- protease binding [IPI]
- protein binding [IPI]
- protein dimerization activity [NAS]
- protein homodimerization activity [IDA, NAS]
- serine-type endopeptidase activity [IDA]
- serine-type peptidase activity [IDA, IMP, NAS]
Gene Ontology Cellular Component
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins.
Tetraspanins, a superfamily of membrane proteins, mediate diverse biological processes through tetraspanin-enriched microdomains in the plasma membrane. However, how their cell-surface presentation is controlled remains unclear. To identify the regulators of tetraspanin trafficking, we conduct sequential genome-wide loss-of-function CRISPR-Cas9 screens based on cell-surface expression of a tetraspanin member, TSPAN8. Several genes potentially involved in endoplasmic reticulum (ER) targeting, different biological ... [more]
Throughput
- High Throughput
Ontology Terms
- phenotype: protein/peptide accumulation (APO:0000149)
Additional Notes
- CRISPR GI screen
- Cell Line: MEC
- Experimental Setup: Timecourse
- GIST: A-phenotypic positive genetic interaction
- Library: Human Two Plasmid Activity-Optimized CRISPR Knockout Library (ADDGENE:1000000095)
- Significance Threshold: Candidate target genes with p < 0.01 from the rescue CRISPR-Cas9 screen on MEC SPPL3 KO cells
Curated By
- BioGRID