Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Fibril treatment changes protein interactions of tau and ?-synuclein in human neurons.

Griffin TA, Schnier PD, Cleveland EM, Newberry RW, Becker J, Carlson GA

In several neurodegenerative disorders, the neuronal proteins tau and ?-synuclein adopt aggregation-prone conformations capable of replicating within and between cells. To better understand how these conformational changes drive neuropathology, we compared the interactomes of tau and ?-synuclein in the presence or the absence of recombinant fibril seeds. Human embryonic stem cells with an inducible neurogenin-2 transgene were differentiated into glutamatergic ... [more]

J Biol Chem Mar. 01, 2023; 299(3);102888 [Pubmed: 36634849]

Throughput

  • Low Throughput

Additional Notes

  • 0N4R Tau (P301L) 24 hours post-seeding
  • 0N4R Tau (WT) 24 hours post-seeding
  • 0N4R Tau (WT) 3wks post-seeding
  • BioID2

Curated By

  • BioGRID