BAIT

SNCA

NACP, PARK1, PARK4, PD1
synuclein, alpha (non A4 component of amyloid precursor)
GO Process (45)
GO Function (20)
GO Component (16)

Gene Ontology Biological Process

Homo sapiens
PREY

DDX3X

CAP-Rf, DBX, DDX14, DDX3, HLP2
DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked
GO Process (29)
GO Function (14)
GO Component (6)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Fibril treatment changes protein interactions of tau and ?-synuclein in human neurons.

Griffin TA, Schnier PD, Cleveland EM, Newberry RW, Becker J, Carlson GA

In several neurodegenerative disorders, the neuronal proteins tau and ?-synuclein adopt aggregation-prone conformations capable of replicating within and between cells. To better understand how these conformational changes drive neuropathology, we compared the interactomes of tau and ?-synuclein in the presence or the absence of recombinant fibril seeds. Human embryonic stem cells with an inducible neurogenin-2 transgene were differentiated into glutamatergic ... [more]

J Biol Chem Mar. 01, 2023; 299(3);102888 [Pubmed: 36634849]

Throughput

  • High Throughput

Additional Notes

  • BioID2
  • alpha-Synuclein (A53T) 24 hours post-seeding

Curated By

  • BioGRID