PREY

EEF1D

EF-1D, EF1D, FP1047, UNQ601
eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Unbiased Identification of trans Regulators of ADAR and A-to-I RNA Editing.

Freund EC, Sapiro AL, Li Q, Linder S, Moresco JJ, Yates JR, Li JB

Adenosine-to-inosine RNA editing is catalyzed by adenosine deaminase acting on RNA (ADAR) enzymes that deaminate adenosine to inosine. Although many RNA editing sites are known, few trans regulators have been identified. We perform BioID followed by mass spectrometry to identify trans regulators of ADAR1 and ADAR2 in HeLa and M17 neuroblastoma cells. We identify known and novel ADAR-interacting proteins. Using ... [more]

Cell Rep May. 19, 2020; 31(7);107656 [Pubmed: 32433965]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • High confidence protein hits had fold changes of LFC > 2 in at least two biological replicates.
  • M17 cells

Curated By

  • BioGRID