BAIT
ADARB1
ADAR2, DRABA2, DRADA2, RED1
adenosine deaminase, RNA-specific, B1
GO Process (10)
GO Function (6)
GO Component (4)
Gene Ontology Biological Process
- RNA processing [IDA]
- adenosine to inosine editing [IDA, TAS]
- base conversion or substitution editing [IC]
- gene expression [TAS]
- mRNA modification [TAS]
- negative regulation of cell migration [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of protein kinase activity by regulation of protein phosphorylation [IDA]
- positive regulation of viral genome replication [IMP]
- regulation of cell cycle [IDA]
Gene Ontology Molecular Function
Homo sapiens
PREY
HSPA5
BIP, GRP78, HEL-S-89n, MIF2
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
GO Process (14)
GO Function (10)
GO Component (12)
Gene Ontology Biological Process
- ATP catabolic process [ISS]
- ER-associated ubiquitin-dependent protein catabolic process [TAS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- blood coagulation [TAS]
- cellular protein metabolic process [TAS]
- cellular response to glucose starvation [IDA]
- endoplasmic reticulum unfolded protein response [TAS]
- maintenance of protein localization in endoplasmic reticulum [IMP]
- negative regulation of apoptotic process [IMP, TAS]
- platelet activation [TAS]
- platelet degranulation [TAS]
- positive regulation of cell migration [IMP]
- regulation of protein folding in endoplasmic reticulum [TAS]
- substantia nigra development [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- COP9 signalosome [IDA]
- endoplasmic reticulum [IDA, IMP, TAS]
- endoplasmic reticulum chaperone complex [IDA]
- endoplasmic reticulum lumen [TAS]
- endoplasmic reticulum membrane [TAS]
- endoplasmic reticulum-Golgi intermediate compartment [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- integral component of endoplasmic reticulum membrane [IDA]
- membrane [IDA]
- midbody [IDA]
- nucleus [IDA, IMP]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Unbiased Identification of trans Regulators of ADAR and A-to-I RNA Editing.
Adenosine-to-inosine RNA editing is catalyzed by adenosine deaminase acting on RNA (ADAR) enzymes that deaminate adenosine to inosine. Although many RNA editing sites are known, few trans regulators have been identified. We perform BioID followed by mass spectrometry to identify trans regulators of ADAR1 and ADAR2 in HeLa and M17 neuroblastoma cells. We identify known and novel ADAR-interacting proteins. Using ... [more]
Cell Rep May. 19, 2020; 31(7);107656 [Pubmed: 32433965]
Throughput
- High Throughput
Additional Notes
- BioID
- High confidence protein hits had fold changes of LFC > 2 in at least two biological replicates.
- M17 cells
Curated By
- BioGRID