BAIT

RPT5

YTA1, proteasome regulatory particle base subunit RPT5, L000002555, YOR117W
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

APC11

anaphase promoting complex subunit 11, L000004322, YDL008W
Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
UPS Project
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Yeast Nst1 is a novel component of P-bodies and is a specific suppressor of proteasome base assembly defects.

Cheng CL, Wong MK, Hochstrasser M

Proteasome assembly utilizes multiple dedicated assembly chaperones and is regulated by signaling pathways that respond to diverse stress conditions. To discover new factors influencing proteasome base assembly, we screened a tiled high-copy yeast genomic library to identify dosage suppressors of a temperature-sensitive proteasome regulatory particle (RP) base mutant. The screen identified negative salt tolerance 1 (Nst1), a protein that when ... [more]

Mol Biol Cell Oct. 01, 2021; 32(20);ar6 [Pubmed: 34347506]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: temperature sensitive growth (APO:0000092)

Additional Notes

  • suppression of the strong RPT2,5 PA growth defect at elevated temperature

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPT5 APC11
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3109BioGRID
1952166

Curated By

  • BioGRID