BAIT

TUB3

alpha-tubulin TUB3, L000002389, YML124C
Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

UBA3

NEDD8-activating protein UBA3, L000004373, YPR066W
Protein that activates Rub1p (NEDD8) before neddylation; acts together with Ula1p; may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
UPS Project
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Tubulin isotypes optimize distinct spindle positioning mechanisms during yeast mitosis.

Nsamba ET, Bera A, Costanzo M, Boone C, Gupta ML

Microtubules are dynamic cytoskeleton filaments that are essential for a wide range of cellular processes. They are polymerized from tubulin, a heterodimer of ?- and ?-subunits. Most eukaryotic organisms express multiple isotypes of ?- and ?-tubulin, yet their functional relevance in any organism remains largely obscure. The two ?-tubulin isotypes in budding yeast, Tub1 and Tub3, are proposed to be ... [more]

J Cell Biol Dec. 06, 2020; 220(12); [Pubmed: 34739032]

Throughput

  • High Throughput

Ontology Terms

  • colony size (APO:0000063)

Additional Notes

  • SGA genetic interaction dataset for the loss of TUB3. All reported hits have P value < 0.05. Negative synthetic genetic integrations have score < -0.08, while positive synthetic integrations have score > 0.08.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TUB3 UBA3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1352BioGRID
2160228

Curated By

  • BioGRID