PREY

ARRB2

ARB2, ARR2, BARR2
arrestin, beta 2
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells.

Lobingier BT, Huettenhain R, Eichel K, Miller KB, Ting AY, von Zastrow M, Krogan NJ

Cells operate through protein interaction networks organized in space and time. Here, we describe an approach to resolve both dimensions simultaneously by using proximity labeling mediated by engineered ascorbic acid peroxidase (APEX). APEX has been used to capture entire organelle proteomes with high temporal resolution, but its breadth of labeling is generally thought to preclude the higher spatial resolution necessary ... [more]

Cell Apr. 06, 2017; 169(2);350-360.e12 [Pubmed: 28388416]

Throughput

  • High Throughput|Low Throughput

Additional Notes

  • LTP hits detected by western

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
OPRD1 ARRB2
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID