Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells.

Lobingier BT, Huettenhain R, Eichel K, Miller KB, Ting AY, von Zastrow M, Krogan NJ

Cells operate through protein interaction networks organized in space and time. Here, we describe an approach to resolve both dimensions simultaneously by using proximity labeling mediated by engineered ascorbic acid peroxidase (APEX). APEX has been used to capture entire organelle proteomes with high temporal resolution, but its breadth of labeling is generally thought to preclude the higher spatial resolution necessary ... [more]

Cell Apr. 06, 2017; 169(2);350-360.e12 [Pubmed: 28388416]

Throughput

  • High Throughput

Curated By

  • BioGRID