Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

INKA2, a novel p53 target that interacts with the serine/threonine kinase PAK4.

Liu YY, Tanikawa C, Ueda K, Matsuda K

The p53 protein is a tumour suppressor and transcription factor that regulates the expression of target genes involved in numerous stress responses systems. In this study, we designed a screening strategy using DNA damage?induced mouse and human transcriptome data to identify novel downstream targets of p53. Our method selected genes with an induced expression in multiple organs of X?ray?irradiated p53 ... [more]

Int J Oncol Jun. 01, 2019; 54(6);1907-1920 [Pubmed: 31081062]

Throughput

  • High Throughput

Curated By

  • BioGRID