BAIT

APOBEC3H

A3H, ARP-10, ARP10, RP4-742C19.10-002
apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3H
Homo sapiens
PREY

APOBEC3H

A3H, ARP-10, ARP10, RP4-742C19.10-002
apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3H
Homo sapiens

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The Role of RNA in HIV-1 Vif-Mediated Degradation of APOBEC3H.

Wang J, Becker JT, Shi K, Lauer KV, Salamango DJ, Aihara H, Shaban NM, Harris RS

As many as five members of the APOBEC3 family of DNA cytosine deaminases are capable of inhibiting HIV-1 replication by deaminating viral cDNA cytosines and interfering with reverse transcription. HIV-1 counteracts restriction with the virally encoded Vif protein, which forms a hybrid ubiquitin ligase complex that directly binds APOBEC3 enzymes and targets them for proteasomal degradation. APOBEC3H (A3H) is unique ... [more]

J Mol Biol Dec. 06, 2019; 431(24);5019-5031 [Pubmed: 31628948]

Throughput

  • Low Throughput

Curated By

  • BioGRID