PREY

YWHAE

14-3-3E, HEL2, KCIP-1, MDCR, MDS
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The herpesvirus accessory protein ?134.5 facilitates viral replication by disabling mitochondrial translocation of RIG-I.

Liu X, Ma Y, Voss K, van Gent M, Chan YK, Gack MU, Gale M, He B

RIG-I and MDA5 are cytoplasmic RNA sensors that mediate cell intrinsic immunity against viral pathogens. While it has been well-established that RIG-I and MDA5 recognize RNA viruses, their interactive network with DNA viruses, including herpes simplex virus 1 (HSV-1), remains less clear. Using a combination of RNA-deep sequencing and genetic studies, we show that the ?134.5 gene product, a virus-encoded ... [more]

PLoS Pathog Mar. 01, 2021; 17(3);e1009446 [Pubmed: 33770145]

Throughput

  • Low Throughput

Curated By

  • BioGRID