Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Cross-linking mass spectrometry reveals the structural topology of peripheral NuRD subunits relative to the core complex.
The multi-subunit nucleosome remodeling and deacetylase (NuRD) complex consists of seven subunits, each of which comprises two or three paralogs in vertebrates. These paralogs define mutually exclusive and functionally distinct complexes. In addition, several proteins in the complex are multimeric, which complicates structural studies. Attempts to purify sufficient amounts of endogenous complex or recombinantly reconstitute the complex for structural studies ... [more]
FEBS J May. 01, 2021; 288(10);3231-3245 [Pubmed: 33283408]
Throughput
- High Throughput
Curated By
- BioGRID