BAIT

SPT5

transcription elongation factor SPT5, L000002031, YML010W

Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair

GO Process (8)

GO Function (7)

GO Component (4)

Gene Ontology Biological Process

7-methylguanosine mRNA capping [IGI, IPI]

intracellular mRNA localization [IMP]

mRNA splicing, via spliceosome [IMP]

negative regulation of transcription elongation from RNA polymerase I promoter [IGI]

positive regulation of transcription elongation from RNA polymerase I promoter [IMP]

positive regulation of transcription elongation from RNA polymerase II promoter [IMP]

regulation of rRNA processing [IMP]

regulation of transcription-coupled nucleotide-excision repair [IGI]

Gene Ontology Molecular Function

RNA polymerase I core binding [IPI]
RNA polymerase I transcription factor binding [IPI]
RNA polymerase II core binding [IPI]
core RNA polymerase I binding transcription factor activity [IC]
core RNA polymerase II binding transcription factor activity [IGI, IMP, IPI]
mRNA binding [IDA]
rRNA binding [IDA]

Gene Ontology Cellular Component

DSIF complex [IPI]

mitochondrion [IDA]

nucleus [IDA]

rDNA heterochromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SPT16

CDC68, SSF1, chromatin-remodeling protein SPT16, L000002038, YGL207W

Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; SPT16 specifically required for diauxic shift-induced H2B deposition onto rDNA genes; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome

GO Process (7)

GO Function (3)

GO Component (5)

Gene Ontology Biological Process

DNA replication-independent nucleosome organization [IDA]

DNA-dependent DNA replication [IPI]

chromatin organization involved in regulation of transcription [IMP]

nucleosome assembly [IDA]

positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IDA]

positive regulation of transcription initiation from RNA polymerase II promoter [IGI, IMP]

transcription elongation from RNA polymerase II promoter [IPI]

Gene Ontology Molecular Function

chromatin binding [IDA]
histone binding [IDA]
nucleosome binding [IDA]

Gene Ontology Cellular Component

FACT complex [IGI, IPI]

alpha DNA polymerase:primase complex [IDA]

nuclear chromatin [IDA]

replication fork protection complex [IDA]

transcription elongation factor complex [IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Spt5 histone binding activity preserves chromatin during transcription by RNA polymerase II.

Evrin C, Serra-Cardona A, Duan S, Mukherjee PP, Zhang Z, Labib KPM

Nucleosomes are disrupted transiently during eukaryotic transcription, yet the displaced histones must be retained and redeposited onto DNA, to preserve nucleosome density and associated histone modifications. Here, we show that the essential Spt5 processivity factor of RNA polymerase II (Pol II) plays a direct role in this process in budding yeast. Functional orthologues of eukaryotic Spt5 are present in archaea ... [more]

EMBO J Mar. 01, 2022; 41(5);e109783 [Pubmed: 35102600]

Throughput

Low Throughput

Additional Notes

Figure 1
Spt5N

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SPT5 SPT16	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Evrin C (2022)	Low	-	BioGRID	3547412
SPT16 SPT5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Foltman M (2013)	Low	-	BioGRID	1277089
SPT5 SPT16	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lindstrom DL (2003)	High	-	BioGRID	-
SPT16 SPT5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3597536
SPT16 SPT5	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Han J (2010)	Low	-	BioGRID	-
SPT16 SPT5	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Stevens JR (2011)	Low	-	BioGRID	574044

Curated By

BioGRID

Interaction Summary

SPT5

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase I core binding [IPI]RNA polymerase I transcription factor binding [IPI]RNA polymerase II core binding [IPI]core RNA polymerase I binding transcription factor activity [IC]core RNA polymerase II binding transcription factor activity [IGI, IMP, IPI]mRNA binding [IDA]rRNA binding [IDA]

Gene Ontology Cellular Component

SPT16

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]histone binding [IDA]nucleosome binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

Spt5 histone binding activity preserves chromatin during transcription by RNA polymerase II.

Throughput

Additional Notes

Related interactions

Curated By

chromatin binding [IDA]
histone binding [IDA]
nucleosome binding [IDA]